human glial cell line Search Results


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alomone labs g-240
G 240, supplied by alomone labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai Korain Biotech Co Ltd human glial cell line
Human Glial Cell Line, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio neurotrophic factor gdnf elisa kit
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Neurotrophic Factor Gdnf Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio csb e04565h
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Csb E04565h, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Alomone Labs recombinant gdnf
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Recombinant Gdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene hgdnf cdna
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Hgdnf Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio gdnf picokine elisa kit boster biological technology
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Gdnf Picokine Elisa Kit Boster Biological Technology, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Alomone Labs rhgdnf
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Rhgdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Alomone Labs human gdnf
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Human Gdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human gdnf/product/Alomone Labs
Average 93 stars, based on 1 article reviews
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Alomone Labs neurotrophic factor gdnf
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Neurotrophic Factor Gdnf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Alomone Labs glial cell derived neurotrophic factor
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Glial Cell Derived Neurotrophic Factor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene human gdnf cdna nm 199234
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Human Gdnf Cdna Nm 199234, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Cell Culture, Quantitative RT-PCR, Western Blot

A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics

Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using ELISA, indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor (GDNF) in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.

Journal: Journal of Neural Engineering

Article Title: Enhancing facial nerve regeneration with scaffold-free conduits engineered using dental pulp stem cells and their endogenous, aligned extracellular matrix

doi: 10.1088/1741-2552/ad749d

Figure Lengend Snippet: Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using ELISA, indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor (GDNF) in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.

Article Snippet: Then human BDNF (PicoKine ELISA Kit, Boster Biological Technology) and GDNF (PicoKine ELISA Kit, Boster Biological Technology) ELISA kits were used to measure the NTF concentrations in the lysates.

Techniques: Staining, Light Microscopy, Electron Microscopy, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Immunofluorescence, Produced